Contamination Detection of CHO-Cell-Culture Using Capacitance Spectroscopy and Flow-imaging Microscopy

The combination of the Capacitance spectroscopy and Flow imaging microscopy imaging technology will provide the real-time detection of bacterial contamination in biopharmaceutical manufacturing at the currently established limits of on-line detection. Capacitance spectra and cell morphology data will be incorporated into descriptive and predictive chemometric models (PCA and PLS). These models will establish meaningful correlations between in-line and at-line capacitance and imaging data and the contamination state of the bioreaction. This will substantially reduce the time for the detection of contamination and the resulting cost (resources and labor) associated with a contamination event. The utilization of the technology and predictive modeling will allow facile and faster detection of bacterial contamination in bioprocess manufacturing.