Protective excipients, known as lyoprotectants, safeguard cells during lyophilization to mitigate viability loss, with current research mostly focuses on a few sugar or sugar-derivatives, like trehalose and sucrose. To expand the repertoire of lyoprotectants and customize the formulation for specific strains of interest, herein, we report “growth monitoring” as a novel method for the high-throughput screening of lyoprotectants for bio-engineered Saccharomyces Boulardii, a probiotic yeast strain that promotes gastrointestinal health. Thereafter, leveraging this innovative and efficient screening approach, we expanded the repertoire of lyoprotectants beyond the commonly studied excipients and identified promising candidates. Also, by utilizing the extensive screening data for multivariate analyses, this research offers new insights into the molecular characteristics of effective lyoprotectants, facilitating the discovery of next-generation lyoprotectants.
Learning Objectives:
Upon completion, participant will be able to explain the importance of lyoprotectants to maintain cell survivability upon lyophilization.
Upon completion, participant will be able to understand the novel idea of high-throughput screening of lyoprotectants for live cells.
Upon completion, participant will be able to apply multivariate analysis to study the structure-activity relationships of excipients.
