Dry blood spot sampling with Tasso-M20 device can contribute to decentralized and patient‐centric sample collection at home. For bioanalysis, patients receive the Tasso-M20 device in the mail, collect their own blood in a relatively easy fashion with minimal pain, then mail the samples to the bioanalytical laboratory for analysis. The Tasso-M20 employs dried blood collection using a volumetric absorptive micro-sampling (VAMS), enabling collection of precise volume of blood subsequent analysis, as well as lower cost, easier logistics, and minimal biohazard risk, compared to conventional blood collections in the clinic. While there are challenges related to sample quality, stability, and variability, DBS analysis is particularly challenging for large molecule analytes, such as therapeutic proteins, due to potential stability and extractability issues. Here we demonstrate a hybrid LC-MS/MS method with high accuracy, reproducibility, and recovery for quantitation of a model therapeutic protein in samples collected with Tasso-M20 devices. The accuracy and precision were maintained even after 102 days of ambient storage.
Learning Objectives:
Upon completion, participant will be able to describe Decentralized Patient‐Centric Micro-sampling approach.
Upon completion, participant will be able to understand the extraction process of protein analyte from Tasso-M20 volumetric absorptive micro-sampling device.
Upon completion, participant will be able to understand quantitative hybrid LC-MS/MS for quantifying therapeutic proteins in dried blood sample collected on Tasso-M20 volumetric absorptive micro-sampling devices.
